Nanometre resolution stepping pattern and structure of acto-myosin-5a at high ATP reveals new mechanism for processive translocation
نویسندگان
چکیده
Myosin-5a is a molecular motor that transport cargoes and steps in symmetric hand-over-hand mechanism along actin, with both heads attached most of the time. By tracking 20 nm gold nanoparticles to single head myosin-5a heavy meromyosin-like construct, sub-nanometer precision via interferometric scattering (iSCAT) microscopy, analysis stride-sizes showed peaks corresponding 22, 24, 26, 28, 30, 32 34 actin subunits. Kinetic dwell times determined for different stride sizes at limiting [ATP] changes ADP release rate azimuthally strained myosin-5a.
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2022
ISSN: ['0006-3495', '1542-0086']
DOI: https://doi.org/10.1016/j.bpj.2021.11.1444